e coli o157 h7 mutant Search Results


e coli o157 h7 mutant  (ATCC)
96
ATCC e coli o157 h7 mutant
E Coli O157 H7 Mutant, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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three e coli o157 h7 strains  (ATCC)
97
ATCC three e coli o157 h7 strains
Effect of pO157 on survival of <t>E.</t> <t>coli</t> <t>O157:H7</t> in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.
Three E Coli O157 H7 Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli o157 h7 atcc 43888 mutants  (ATCC)
98
ATCC e coli o157 h7 atcc 43888 mutants
Effect of pO157 on survival of <t>E.</t> <t>coli</t> <t>O157:H7</t> in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.
E Coli O157 H7 Atcc 43888 Mutants, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pathogens  (ATCC)
99
ATCC pathogens
Effect of pO157 on survival of <t>E.</t> <t>coli</t> <t>O157:H7</t> in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.
Pathogens, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli o157 h7  (ATCC)
97
ATCC e coli o157 h7
Effect of pO157 on survival of <t>E.</t> <t>coli</t> <t>O157:H7</t> in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.
E Coli O157 H7, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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property reference e coli strains vs94 o157 h7 luxs mutant  (ATCC)
96
ATCC property reference e coli strains vs94 o157 h7 luxs mutant
Effect of pO157 on survival of <t>E.</t> <t>coli</t> <t>O157:H7</t> in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.
Property Reference E Coli Strains Vs94 O157 H7 Luxs Mutant, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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verotoxin mutant e coli o157 h7  (ATCC)
99
ATCC verotoxin mutant e coli o157 h7
Effect of pO157 on survival of <t>E.</t> <t>coli</t> <t>O157:H7</t> in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.
Verotoxin Mutant E Coli O157 H7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e. coli o157:h7 hfq mutant  (Marinus)
90
Marinus e. coli o157:h7 hfq mutant
Effect of pO157 on survival of <t>E.</t> <t>coli</t> <t>O157:H7</t> in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.
E. Coli O157:H7 Hfq Mutant, supplied by Marinus, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacteria escherichia coli k 12 tsbye e coli o157 h7 atcc 43889 tsbye e coli o157 h7 edl 933 mutant  (ATCC)
95
ATCC bacteria escherichia coli k 12 tsbye e coli o157 h7 atcc 43889 tsbye e coli o157 h7 edl 933 mutant
Effect of pO157 on survival of <t>E.</t> <t>coli</t> <t>O157:H7</t> in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.
Bacteria Escherichia Coli K 12 Tsbye E Coli O157 H7 Atcc 43889 Tsbye E Coli O157 H7 Edl 933 Mutant, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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o157 h7 knockout mutant edl933 δgaletkm  (ATCC)
99
ATCC o157 h7 knockout mutant edl933 δgaletkm
Effect of pO157 on survival of <t>E.</t> <t>coli</t> <t>O157:H7</t> in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.
O157 H7 Knockout Mutant Edl933 δgaletkm, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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listeria innocua seeliger  (ATCC)
98
ATCC listeria innocua seeliger
Effect of pO157 on survival of <t>E.</t> <t>coli</t> <t>O157:H7</t> in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.
Listeria Innocua Seeliger, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli castellani and chalmers  (ATCC)
99
ATCC escherichia coli castellani and chalmers
Effect of pO157 on survival of <t>E.</t> <t>coli</t> <t>O157:H7</t> in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.
Escherichia Coli Castellani And Chalmers, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of pO157 on survival of E. coli O157:H7 in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.

Journal:

Article Title: Characterization of an Escherichia coli O157:H7 Plasmid O157 Deletion Mutant and Its Survival and Persistence in Cattle ▿

doi: 10.1128/AEM.02643-06

Figure Lengend Snippet: Effect of pO157 on survival of E. coli O157:H7 in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.

Article Snippet: Three E. coli O157:H7 strains were used in this study: strain ATCC 43894, a human stool isolate producing Shiga toxin types 1 and 2 (referred to as the WT); strain 277, an isogenic strain with the 92-kb plasmid pO157 deleted (referred to as the ΔpO157 mutant); and the complemented strain ΔpO157 mutant 277::pO157Δ ehxA (referred to as the complement) ( 52 ).

Techniques: Mutagenesis, Concentration Assay

Effect of pO157 on survival of E. coli O157:H7 in cattle following oral administration of bacteria. Four steers (steers 1 to 4) were given a single oral dose of 1.0 ×1010 CFU containing both the WT and the ΔpO157 mutant. RAMS samples were cultured by direct plating onto SMAC-CTMV, and sorbitol-negative MUG-negative colonies were confirmed to be the O157 serotype by latex agglutination. At least 15 isolates from each sample were subcultured and differentiated as the WT or the ΔpO157 mutant by PCR. Bar heights represent to total number of E. coli O157:H7 isolates recovered from the steer expressed as log CFU/swab. The proportion of isolates that were either the WT (open white bars) or the ΔpO157 mutant (dotted bars) is shown. Asterisks indicate significant differences between the WT and the ΔpO157 mutant (day 1, P < 0.001; day 3, P < 0.05).

Journal:

Article Title: Characterization of an Escherichia coli O157:H7 Plasmid O157 Deletion Mutant and Its Survival and Persistence in Cattle ▿

doi: 10.1128/AEM.02643-06

Figure Lengend Snippet: Effect of pO157 on survival of E. coli O157:H7 in cattle following oral administration of bacteria. Four steers (steers 1 to 4) were given a single oral dose of 1.0 ×1010 CFU containing both the WT and the ΔpO157 mutant. RAMS samples were cultured by direct plating onto SMAC-CTMV, and sorbitol-negative MUG-negative colonies were confirmed to be the O157 serotype by latex agglutination. At least 15 isolates from each sample were subcultured and differentiated as the WT or the ΔpO157 mutant by PCR. Bar heights represent to total number of E. coli O157:H7 isolates recovered from the steer expressed as log CFU/swab. The proportion of isolates that were either the WT (open white bars) or the ΔpO157 mutant (dotted bars) is shown. Asterisks indicate significant differences between the WT and the ΔpO157 mutant (day 1, P < 0.001; day 3, P < 0.05).

Article Snippet: Three E. coli O157:H7 strains were used in this study: strain ATCC 43894, a human stool isolate producing Shiga toxin types 1 and 2 (referred to as the WT); strain 277, an isogenic strain with the 92-kb plasmid pO157 deleted (referred to as the ΔpO157 mutant); and the complemented strain ΔpO157 mutant 277::pO157Δ ehxA (referred to as the complement) ( 52 ).

Techniques: Mutagenesis, Cell Culture, Agglutination

E. coli O157:H7 carriage in cattle given a single oral dose of the WT or the ΔpO157 mutant

Journal:

Article Title: Characterization of an Escherichia coli O157:H7 Plasmid O157 Deletion Mutant and Its Survival and Persistence in Cattle ▿

doi: 10.1128/AEM.02643-06

Figure Lengend Snippet: E. coli O157:H7 carriage in cattle given a single oral dose of the WT or the ΔpO157 mutant

Article Snippet: Three E. coli O157:H7 strains were used in this study: strain ATCC 43894, a human stool isolate producing Shiga toxin types 1 and 2 (referred to as the WT); strain 277, an isogenic strain with the 92-kb plasmid pO157 deleted (referred to as the ΔpO157 mutant); and the complemented strain ΔpO157 mutant 277::pO157Δ ehxA (referred to as the complement) ( 52 ).

Techniques: